The Quebrada de Humahuaca is a narrow mountain valley in the province of Jujuy, located in the northwest of Argentina. It is about 1,649 kilometers (1,025 miles) north of Buenos Aires. The valley is approximately 155 kilometers (96 miles) long and runs in a north-south direction. It is bordered by the Altiplano to the west and north, by the Sub-Andean hills to the east, and by the warm valleys (Valles Templados) to the south.

The word quebrada means "broken" and refers to a deep valley or ravine. The name comes from Humahuaca, a small city with about 11,000 people. The Grande River (Río Grande) flows through the valley during the summer but is dry in the winter.

This region has been an important place for travel and trade for thousands of years. People have lived there for at least 10,000 years, as shown by remains from early hunter-gatherers. Many stone-walled agricultural terraces, built more than 1,500 years ago, are still used today. These terraces are connected by a field system that links a series of fortified towns called pucaras. Later, the valley was used as a road by the Inca Empire in the 15th century. It also became an important route between the Viceroyalty of the Río de la Plata and the Viceroyalty of Peru. The area was also a site for battles during the Spanish War of Independence.

In 2000, the Quebrada de Humahuaca was declared a protected landscape. It was added to the UNESCO World Heritage List on July 2, 2003.